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aim 2  (Cell Signaling Technology Inc)


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Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Structured Review

    Cell Signaling Technology Inc aim 2
    Aim 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aim+2/AIM2+Rabbit+mAb/10__1097_slash_ccm__0000000000007088-81-61-64
    Average 95 stars, based on 63 article reviews
    aim 2 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Binding Assay:

    Article Title: Interleukin-6-Associated Pyroptosis, Apoptosis, and Necroptosis via JAKs/STAT3-RIPK1 Axis: A Potential Mechanism for CD4+ T-Cell Depletion in Bacterial Sepsis
    Article Snippet: Proteins from purified CD4+ T cells were extracted, quantified using the bicinchoninic acid assay (BCA) protein assay kit (Beyotime, Shanghai, China), separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and transferred to polyvinylidene fluoride membranes. .. Nonspecific binding was blocked with 5% nonfat milk, followed by overnight incubation at 4°C with primary antibodies: Caspase-1 (No. 3866, CST), Caspase-8 (No. 4790, CST), RIPK3 (No. 10188, CST), Caspase-3 (No. 14220, CST), BCL-2 (No. 3498, CST), MLKL (No. 14993, CST), ASC (No. 78000, CST), p-MLKL (No. 91689, CST), RIPK1 (A19580, ABclonal), p-RIPK1 (AP1230, ABclonal), STAT3 (A22434, ABclonal), p-STAT3 (AP0705, ABclonal), AIM-2 (No. 12948, CST), and ZBP-1 (No. 60968, CST). .. Membranes were incubated with HRP-conjugated secondary antibodies (1:3000, GB23303, Servicebio), and band densitometry was analyzed using ImageJ (National Institutes of Health, Bethesda, MD).

    Incubation:

    Article Title: Interleukin-6-Associated Pyroptosis, Apoptosis, and Necroptosis via JAKs/STAT3-RIPK1 Axis: A Potential Mechanism for CD4+ T-Cell Depletion in Bacterial Sepsis
    Article Snippet: Proteins from purified CD4+ T cells were extracted, quantified using the bicinchoninic acid assay (BCA) protein assay kit (Beyotime, Shanghai, China), separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and transferred to polyvinylidene fluoride membranes. .. Nonspecific binding was blocked with 5% nonfat milk, followed by overnight incubation at 4°C with primary antibodies: Caspase-1 (No. 3866, CST), Caspase-8 (No. 4790, CST), RIPK3 (No. 10188, CST), Caspase-3 (No. 14220, CST), BCL-2 (No. 3498, CST), MLKL (No. 14993, CST), ASC (No. 78000, CST), p-MLKL (No. 91689, CST), RIPK1 (A19580, ABclonal), p-RIPK1 (AP1230, ABclonal), STAT3 (A22434, ABclonal), p-STAT3 (AP0705, ABclonal), AIM-2 (No. 12948, CST), and ZBP-1 (No. 60968, CST). .. Membranes were incubated with HRP-conjugated secondary antibodies (1:3000, GB23303, Servicebio), and band densitometry was analyzed using ImageJ (National Institutes of Health, Bethesda, MD).



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